What Is ProteaseTag?
This is a test that can be used to quantify Nutrophil Elastase levels in patients. Combined with specialized immunodection step, the test gives selective quantitative measurement of NE levels in patients.
How Does ProteaseTag Work?
This test can help to detect different respiratory diseases like cystic fibrosis and chronic obstructive pulmonary disease. Apparently, there are cycles that these diseases go through that can lead to constant infection as well as inflammation. The diseases are extremely dangerous, making ProteaseTag a very important test for people who could potentially be suffering.
Other signs of these diseases could be difficulty in breathing and improper or poor functioning of the lungs. Before a doctor can prescribe the correct antibiotics, they first have to be able to test properly. ProteaseTag is ideal for testing. In either case of either CF or COPD, there is a specific type of protease secreted by neutrophils, has been proven to detect anything that is secreted and causes inflammation and infection.
The serious detection of NE is important to keep an accurate measure of the state of a patient, and monitor the status of the disease. It works by correlating the occurrence of unwanted episodes and the current activity of the disease. ProteaseTag uses proven methods of quantitation of currently active proteases. It’s based on chromogenic or fluorogenic peptides that can be broken down. Apparently, there are other tests, but they are very inaccurate vs. Protease tag.
Other tests unlike Protease Tag are known as immunoassays and they can’t distinguish between inactive or active enzymes produced by the diseases, therefore they are considered completely inaccurate. They can’t test the severity of the disease or where its current state is. On the other hand, the Active NE Protease Tag Test Kit has been proven to accurately detect and show levels of NE levels.
ProteaseTag is designed to not only inhibit, but also bind to exact proteases. After they are bound a bridge is formed to a support that will allow them to be detected and included in an immunoassay format to give exact, quantification for accurate measurement and status of the disease.
The ProteaseTag Active NE Immunoassay, will actively measure NE. It also has an additional antibody step during the test that gives more information for an even more accurate measurement of the current status of the disease. There is an increase in sensitivity to detection of the diseases and their current state. This works through a signal amplification that gives even more sensitivity.
The detection occurs through ELISA-type format that will give the tester accurate results in less than 3 hours. NE-Tag uses a detection method that is quantitative and at the same time discriminatory. The Assay kit is said to be accurate at detecting and measuring different aspects of the disease like Sputum Sol, Bronchoalveolar lavage, wound exudate, serum free cell culture.
Before the test or assay, sputum samples had to be collected from people who had CF and then processed. The standards as well as samples had to be kept on ice afterwards before they could add the microplate well.
Apparently, this often led to tests being inaccurate and samples becoming diluted while the plate was being prepped. This is according to the results and reports by the manufacturers of different tests in the past.
ProteaseTag Review Summary
The ProteaseTag gives one of the most accurate NE Immunoassays and provides samples that work for quick assessing active neutrophil elastase levels.
This means apparently, that ProAxis, Protease Tag gives is one of the most accurate tests you a doctor can use to test a patient who has CF or COPD and accurately test their current levels to see how they can be treated best. The kit is proven and is ideal for anyone who tests patients with these diseases.
